Core Facility

The BD FACSAria III sorter is a superior multicolor performance, and legendary ease-of-use that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allow the system to achieve unrivaled sensitivity and resolution. BD FACSAria III cell sorter with a 5 laser 11 colour setup. The BD FACSAria III system has a throughput of 70,000 events per second and can do 4-way sorting and single cell sorting. Wavelength choices include 561-nm (Y/G), as well as the 488-nm (Blue), 633-nm (Red), 405-nm (Violet), and 375-nm (UV) lasers.

BD FACS celesta is a multi-laser flow cytometer with 3 laser and 12 colour setup for delivering high sensitivity and performance. All configurations have blue (488-nm) and violet (405-nm) lasers, which has been paired with a yellow-green (561-nm) laser, for your application needs.

The BD FACS Calibur platform allows users to perform both cell analysis in a single benchtop system. This has 2 lasers, 488nm (Blue) and 633nm (Red), 4 colour system and is routinely used for intracellular and surface markers analyses.

The Flow Cytometry core aims to conduct regular workshops related to multicolor flow sorting and cell analysis for human resource development in state-of-the-art flow cytometry techniques and provides support to various departments in selecting appropriate antibodies panels and experimental designs. An offline workstation with a FlowJo license is also available and networked for data sharing and post-acquisition data analysis.

The Leica DMI6000B is an inverted fluorescence microscope comprising of 6 interchangeable filters for detecting various fluorochromes. It has two independent cameras – DFC295 for high resolution bright-field imaging and DFC360 FX for high frame rate fluorescence imaging. It is also equipped with a fluorescence intensity manager and programmable function keys for easy access to functions.

Leica DMIL (upright) and Leica DMI1000 (inverted) are available for users to perform routine light microscopy imaging. Both microscopes are provided with an interchangeable Leica DFC290 camera for high resolution bright-field imaging. The Leica DMI1000 is also installed in the tissue culture facilities of individual labs and the Core tissue culture area.

A Carl ZiessAxiovert 40 CFL equipped with 3 filters (DAPI, FITC and TRITC) for routine fluorescence imaging is available, along with a ProgRes C3 camera module for image acquisition.

The Olympus FV1000 confocal system comprises a motorized microscope with z focus drift compensation facility for bright field, differential interference contrast and fluorescence imaging with motorized XY scanning stage and CO2 incubation facility for live cell imaging. It is equipped with the following lasers - 405nm, Multi-Argon (458nm, 488nm and 515nm), 559nm and 635nm. Apart from regular confocal imaging, this microscope can be used to perform Multi- Area Time Lapse, FRET, FRAP, FLIM and diffusion experiments.

The FV1000MPE is an upright multiphoton laser-scanning microscope coupled with a Mai Tai HP-Deep See–OL laser with automated broadband wavelength tuning from 690 to 1040nm for deep tissue imaging.

The Imaging Core Facility conducts training sessions regularly for both first time and experienced users. The training sessions comprise of specifically designed modules which include theory and practical sessions. The final authorization is given to the user upon successfully completing the required modules. The hands-on training sessions are tailored to the specific application requirement of each user so that they get the maximum benefit out of these systems. Apart from in-house training, the imaging core organizes sessions by application specialists from Leica and Olympus. Till date, 2 sessions for the Olympus FV1000 confocal microscope and 3 sessions for the Leica DMI6000B were conducted.

Genetic Analyzer 3130 is a 4 capillary series system with Electroosmotic flow suppression polymers (EOF). This system gives you all the advance automation with hands free operation and superior performance. This system provides compatibility with the existing application software systems, long-term reliability, automated polymer delivery system, enhanced thermal control, and optimized for multiple application.

QuantStudio 12 K Flex system is designed for maximum throughput, outstanding flexibility with 5-interchangeable blocks, scalability and user friendly. This system is widely used in gene expression analysis, SNP genotyping, copynumber analysis, digital PCR technology, Micro RNA and other noncoding RNA analysis.
QuantStudio 6 K Flex system facilitates high-throughput analysis with 2-interchangeable blocks. This system is commonly used for PCR applications such as gene expression, gene regulation, gene variation and protein expression.

The Radioactivity Core Facility provides researchers a secure access to radiolabelled isotopes and instrumentation for detecting radioactivity. The facility currently has Greiger counters, GE Storm 365 Phosphor imager and a Perkin Elmer Tricarb Liquid Scintillation Counter.

Alcian Blue, Perl’s Prussian Blue Iron Stain, Periodic Acid Schiff, Masson Trichrome, Gordon Sweet Reticulin, Acid Fast Bacillus stain for Mycobacterium Tuberculosis, Toluidine Blue, Masson Fontana, Verhoeff’s elastic stain, Sirus Red stain, Hematoxylin and Eosin (H&E), Safaranin-O, Elastic van Gieson, Paraffin blocks and tissue mounting, Unstained slide sections for specific immunostaining.

Cytospin preparation, Cell block preparation, Cryostate sectioning.